| 1. | Effect of electrical and chemical activation on reconstructed embryo formed by rat - mouse inter - species somatic cell nuclear transplantation
小鼠种间体细胞核移植重组胚发育的影响 |
| 2. | 2 ) the rat - mouse reconstructed embryos activated were been cultured in the maturation mediums of tcm199 , ml6 , czb and czbm ( czb modified ) for 48h
直接注射法和融合法构建的重组胚桑椹胚发育率分别可达9 . 84 、 9 . 48 。 |
| 3. | 6 - dmap + ccb + ethanol can effectively activate the reconstructed rat - mouse embryos . 5 ) 225 reconstructed embryos from 2 - cell to morula were transferred into 46 recipients
23 l6 dmap乙醇ccb能有效地激活种间核移植重构胚。 |
| 4. | 2 ) we got 55 . 08 % of fusion rate when reconstructed embryos were fused with 1500 - 1800v / cm voltage and 50ms impulse . in addition , a same result can be obtained with low voltage + length impulse or strong voltage + short impulse
41的卵母细胞达到成熟：在1500 1800v cm ， 30 40sin条件下，大鼠牛核移植重构胚能达到50厂0 55 |
| 5. | From more than 430 attempted fusions , 277 reconstructed embryos were made in this way ; of these , only 29 survived to the stage that they could be returned to foster mothers , and only one survived to term
在所尝试的430多次的细胞合成中，有277个重组的胚胎是以这种方式获得的；其中，只有29个存活到能被植入养母子宫的阶段，最后能足月分娩的只剩一个了。 |
| 6. | The enucleated rates can reach at 87 . 06 % . 3 ) three activated methods may effectively activate the black bear - cat reconstructed embryos . whereas activated effect of ethanol + 6 - dmap + ccb is more effective , the cleavage rate and the development rate to m
重组胚在3种培养液中的发育能力有差异t 0刀引， m199成熟培养液比dmem12 、 m16更能支待重组胚胎的卵裂和桑棋胚发育，卵裂率和桑堪胚发育率分别为63 |
| 7. | This study were designed to investigate development potency of the reconstructed embryos of black bear - cat inter - species nuclear transfer in vitro / vivo , to develop the principle and technology of somatic nuclear transplantation in inter - species of mammalian and to study the mechanism of coordination between cell nuclear and cytoplasm . somatic nuclear transplantation technology has been used to establish stem cell from different animals , which can provide materials to treat various diseases
本研究运用体细胞核移植的原理和技术，以六月龄雌性黑熊的成纤维细胞为供核体，去核未受精家猫成熟卵母细胞为受体，构建种间核移植重组胚胎，研究种问体细胞核移植胚胎的构建方法和重组胚胎的体内外发育潜能及其影响因素。 |
| 8. | In order to establish the technique of isolating es cell , and test the developmental ability of reconstructed embryos derived from es cell and oocytes of intra - or inter - species , mouse es cell were isolated from mouse blastocysts , and were used as donor nuclear , and were transferred into oocytes of various strains . the factors that influenced the development of reconstructed oocytes were determined . the developmental ability of embryos reconstructed with mouse es cells and rabbit oocytes was observed and analyzed
为了建立胚胎干细胞分离技术及了解其在同种和异种卵母细胞中的发育能力，首先从小鼠囊胚分离胚胎干细胞，并以它作为核供体，移植到同种不同品系去核的小鼠卵母细胞内，观察不同品系的卵母细胞对胚胎干细胞重构胚发育的影响。 |
| 9. | This study were designed to investigate development potency of the reconstructed embryos of rat - mouse and rat - bovine inter - species nuclear transfer in vitro / vivo , to develop the principle and technology of somatic nuclear transplantation in inter - species of mammalian and to study the mechanism of coordination between cell nuclear and cytoplasm . somatic nuclear transplantation technology has been used to establish stem cell from different animals , which can provide material to treat various diseases
本研究运用体细胞核移植的原理和技术，以成年大鼠的成纤维细胞为供体，去核小鼠和牛成熟卵母细胞为受体，构建种间核移植重组胚胎，研究种间体细胞核移植胚胎的构建方法和重组胚胎的体内外发育潜能及其影响因素。 |
| 10. | Recovery of microtubule organizing centers in enucleated oocytes and condensation of chromosome and organization of spindle in reconstructed embryos were analysed . a non - invasive method of enucleation mouse oocytes has been developed . firstly , the progression of mouse oocytes in meitosis i was ascertained by immunofluoresence technique
另外，本试验通过在m16基础上添加eaa 、 non - eaa和葡萄糖显著提高了胚胎的桑椹率、囊胚率和孵化率；单独添加葡萄糖的培养液，其桑椹率显著升高，但囊胚率和孵化率的升高并不显著，所以胚胎培养液中氨基酸和葡萄糖能协同促进胚胎发育。 |
